anti il 33 neutralizing antibody Search Results


il 17a neutralizing antibody  (BPS Bioscience)
91
BPS Bioscience il 17a neutralizing antibody
Impact of <t>IL-17A</t> on MNs: ( A ) the MAP-2 immunostaining after different concentrations (5 ng/mL, 50 ng/mL, and 500 ng/mL) of IL-17A-stimulated MNs for three days; ( B ) the viability of MNs under the same IL-17A treatment for three days was evaluated using CCK-8 assay; ( C ) the MAP-2 positive MNs, counting manually in different IL-17A treatments; ( D ) the neurite length of MAP-2 positive MNs was calculated using Neuron J in the FIJI software; ( E ) the immunostaining of MAP-2 with the same IL-17A treatment at different times (three days and six days); ( F ) the MAP-2 positive MNs’ counting in IL-17A treatment at different times; ( G ) the length of the neurite was measured under the same treatment. * p < 0.05, ** p < 0.01, *** p < 0.001 versus controls, ns: no significance.
Il 17a Neutralizing Antibody, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+il+33+neutralizing+antibody/Anti-IL-17A+Neutralizing+Antibody/pmc08348495-140-4-8
Average 91 stars, based on 1 article reviews
il 17a neutralizing antibody - by Bioz Stars, 2026-10
91/100 stars
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neutralizing rat igg1 anti-mouse il-4 mab 11b11  (Verax Inc)
90
Verax Inc neutralizing rat igg1 anti-mouse il-4 mab 11b11
Impact of <t>IL-17A</t> on MNs: ( A ) the MAP-2 immunostaining after different concentrations (5 ng/mL, 50 ng/mL, and 500 ng/mL) of IL-17A-stimulated MNs for three days; ( B ) the viability of MNs under the same IL-17A treatment for three days was evaluated using CCK-8 assay; ( C ) the MAP-2 positive MNs, counting manually in different IL-17A treatments; ( D ) the neurite length of MAP-2 positive MNs was calculated using Neuron J in the FIJI software; ( E ) the immunostaining of MAP-2 with the same IL-17A treatment at different times (three days and six days); ( F ) the MAP-2 positive MNs’ counting in IL-17A treatment at different times; ( G ) the length of the neurite was measured under the same treatment. * p < 0.05, ** p < 0.01, *** p < 0.001 versus controls, ns: no significance.
Neutralizing Rat Igg1 Anti Mouse Il 4 Mab 11b11, supplied by Verax Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+il+33+neutralizing+antibody/rat+anti+mouse+il+4+neutralizing+antibody/pm12370375-39-32-37
Average 90 stars, based on 1 article reviews
neutralizing rat igg1 anti-mouse il-4 mab 11b11 - by Bioz Stars, 2026-10
90/100 stars
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purified neutralizing monoclonal rat anti-mouse il-6 igg monoclonal antibody (anti-il-6 mab)  (Becton Dickinson)
90
Becton Dickinson purified neutralizing monoclonal rat anti-mouse il-6 igg monoclonal antibody (anti-il-6 mab)
Impact of <t>IL-17A</t> on MNs: ( A ) the MAP-2 immunostaining after different concentrations (5 ng/mL, 50 ng/mL, and 500 ng/mL) of IL-17A-stimulated MNs for three days; ( B ) the viability of MNs under the same IL-17A treatment for three days was evaluated using CCK-8 assay; ( C ) the MAP-2 positive MNs, counting manually in different IL-17A treatments; ( D ) the neurite length of MAP-2 positive MNs was calculated using Neuron J in the FIJI software; ( E ) the immunostaining of MAP-2 with the same IL-17A treatment at different times (three days and six days); ( F ) the MAP-2 positive MNs’ counting in IL-17A treatment at different times; ( G ) the length of the neurite was measured under the same treatment. * p < 0.05, ** p < 0.01, *** p < 0.001 versus controls, ns: no significance.
Purified Neutralizing Monoclonal Rat Anti Mouse Il 6 Igg Monoclonal Antibody (Anti Il 6 Mab), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+il+33+neutralizing+antibody/purified+neutralizing+monoclonal+rat+anti+mouse+il+6+igg+monoclonal+antibody++anti+il+6+mab+/pmc03067852-81-22-31
Average 90 stars, based on 1 article reviews
purified neutralizing monoclonal rat anti-mouse il-6 igg monoclonal antibody (anti-il-6 mab) - by Bioz Stars, 2026-10
90/100 stars
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anti-il-17a neutralizing antibodies  (RD Biotech)
90
RD Biotech anti-il-17a neutralizing antibodies
Impact of <t>IL-17A</t> on MNs: ( A ) the MAP-2 immunostaining after different concentrations (5 ng/mL, 50 ng/mL, and 500 ng/mL) of IL-17A-stimulated MNs for three days; ( B ) the viability of MNs under the same IL-17A treatment for three days was evaluated using CCK-8 assay; ( C ) the MAP-2 positive MNs, counting manually in different IL-17A treatments; ( D ) the neurite length of MAP-2 positive MNs was calculated using Neuron J in the FIJI software; ( E ) the immunostaining of MAP-2 with the same IL-17A treatment at different times (three days and six days); ( F ) the MAP-2 positive MNs’ counting in IL-17A treatment at different times; ( G ) the length of the neurite was measured under the same treatment. * p < 0.05, ** p < 0.01, *** p < 0.001 versus controls, ns: no significance.
Anti Il 17a Neutralizing Antibodies, supplied by RD Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+il+33+neutralizing+antibody/anti+il+17a+neutralizing+antibodies/pmc09523421-39-54-59
Average 90 stars, based on 1 article reviews
anti-il-17a neutralizing antibodies - by Bioz Stars, 2026-10
90/100 stars
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neutralizing anti-human il-7 antibody  (Becton Dickinson)
90
Becton Dickinson neutralizing anti-human il-7 antibody
Impact of <t>IL-17A</t> on MNs: ( A ) the MAP-2 immunostaining after different concentrations (5 ng/mL, 50 ng/mL, and 500 ng/mL) of IL-17A-stimulated MNs for three days; ( B ) the viability of MNs under the same IL-17A treatment for three days was evaluated using CCK-8 assay; ( C ) the MAP-2 positive MNs, counting manually in different IL-17A treatments; ( D ) the neurite length of MAP-2 positive MNs was calculated using Neuron J in the FIJI software; ( E ) the immunostaining of MAP-2 with the same IL-17A treatment at different times (three days and six days); ( F ) the MAP-2 positive MNs’ counting in IL-17A treatment at different times; ( G ) the length of the neurite was measured under the same treatment. * p < 0.05, ** p < 0.01, *** p < 0.001 versus controls, ns: no significance.
Neutralizing Anti Human Il 7 Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+il+33+neutralizing+antibody/neutralizing+human+anti+il+6+antibody/pmc02659466-73-29-38
Average 90 stars, based on 1 article reviews
neutralizing anti-human il-7 antibody - by Bioz Stars, 2026-10
90/100 stars
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anti-human il-10 neutralizing antibody  (Petrotech Inc)
90
Petrotech Inc anti-human il-10 neutralizing antibody
Impact of <t>IL-17A</t> on MNs: ( A ) the MAP-2 immunostaining after different concentrations (5 ng/mL, 50 ng/mL, and 500 ng/mL) of IL-17A-stimulated MNs for three days; ( B ) the viability of MNs under the same IL-17A treatment for three days was evaluated using CCK-8 assay; ( C ) the MAP-2 positive MNs, counting manually in different IL-17A treatments; ( D ) the neurite length of MAP-2 positive MNs was calculated using Neuron J in the FIJI software; ( E ) the immunostaining of MAP-2 with the same IL-17A treatment at different times (three days and six days); ( F ) the MAP-2 positive MNs’ counting in IL-17A treatment at different times; ( G ) the length of the neurite was measured under the same treatment. * p < 0.05, ** p < 0.01, *** p < 0.001 versus controls, ns: no significance.
Anti Human Il 10 Neutralizing Antibody, supplied by Petrotech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+il+33+neutralizing+antibody/anti+human+il+10+neutralizing+antibody/pmc04259327-40-3-9
Average 90 stars, based on 1 article reviews
anti-human il-10 neutralizing antibody - by Bioz Stars, 2026-10
90/100 stars
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neutralizing anti-il-1ra polyclonal antibody  (GeneTex)
90
GeneTex neutralizing anti-il-1ra polyclonal antibody
Impact of <t>IL-17A</t> on MNs: ( A ) the MAP-2 immunostaining after different concentrations (5 ng/mL, 50 ng/mL, and 500 ng/mL) of IL-17A-stimulated MNs for three days; ( B ) the viability of MNs under the same IL-17A treatment for three days was evaluated using CCK-8 assay; ( C ) the MAP-2 positive MNs, counting manually in different IL-17A treatments; ( D ) the neurite length of MAP-2 positive MNs was calculated using Neuron J in the FIJI software; ( E ) the immunostaining of MAP-2 with the same IL-17A treatment at different times (three days and six days); ( F ) the MAP-2 positive MNs’ counting in IL-17A treatment at different times; ( G ) the length of the neurite was measured under the same treatment. * p < 0.05, ** p < 0.01, *** p < 0.001 versus controls, ns: no significance.
Neutralizing Anti Il 1ra Polyclonal Antibody, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+il+33+neutralizing+antibody/neutralizing+anti+il+1ra+polyclonal+antibody/pmc02516901-42-1-10
Average 90 stars, based on 1 article reviews
neutralizing anti-il-1ra polyclonal antibody - by Bioz Stars, 2026-10
90/100 stars
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mouse il-22 antibody  (Bio-Techne corporation)
93
Bio-Techne corporation mouse il-22 antibody
Impact of <t>IL-17A</t> on MNs: ( A ) the MAP-2 immunostaining after different concentrations (5 ng/mL, 50 ng/mL, and 500 ng/mL) of IL-17A-stimulated MNs for three days; ( B ) the viability of MNs under the same IL-17A treatment for three days was evaluated using CCK-8 assay; ( C ) the MAP-2 positive MNs, counting manually in different IL-17A treatments; ( D ) the neurite length of MAP-2 positive MNs was calculated using Neuron J in the FIJI software; ( E ) the immunostaining of MAP-2 with the same IL-17A treatment at different times (three days and six days); ( F ) the MAP-2 positive MNs’ counting in IL-17A treatment at different times; ( G ) the length of the neurite was measured under the same treatment. * p < 0.05, ** p < 0.01, *** p < 0.001 versus controls, ns: no significance.
Mouse Il 22 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+il+33+neutralizing+antibody/Mouse+IL-22+Antibody/custom%40af582%4030097293
Average 93 stars, based on 1 article reviews
mouse il-22 antibody - by Bioz Stars, 2026-10
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mouse il-17/il-17a antibody  (Bio-Techne corporation)
93
Bio-Techne corporation mouse il-17/il-17a antibody
Impact of <t>IL-17A</t> on MNs: ( A ) the MAP-2 immunostaining after different concentrations (5 ng/mL, 50 ng/mL, and 500 ng/mL) of IL-17A-stimulated MNs for three days; ( B ) the viability of MNs under the same IL-17A treatment for three days was evaluated using CCK-8 assay; ( C ) the MAP-2 positive MNs, counting manually in different IL-17A treatments; ( D ) the neurite length of MAP-2 positive MNs was calculated using Neuron J in the FIJI software; ( E ) the immunostaining of MAP-2 with the same IL-17A treatment at different times (three days and six days); ( F ) the MAP-2 positive MNs’ counting in IL-17A treatment at different times; ( G ) the length of the neurite was measured under the same treatment. * p < 0.05, ** p < 0.01, *** p < 0.001 versus controls, ns: no significance.
Mouse Il 17/Il 17a Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+il+33+neutralizing+antibody/Mouse+IL-17%2FIL-17A+Antibody/bio-techne+corporation___mab421
Average 93 stars, based on 1 article reviews
mouse il-17/il-17a antibody - by Bioz Stars, 2026-10
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human il-5 r alpha/cd125 antibody  (Bio-Techne corporation)
90
Bio-Techne corporation human il-5 r alpha/cd125 antibody
Impact of <t>IL-17A</t> on MNs: ( A ) the MAP-2 immunostaining after different concentrations (5 ng/mL, 50 ng/mL, and 500 ng/mL) of IL-17A-stimulated MNs for three days; ( B ) the viability of MNs under the same IL-17A treatment for three days was evaluated using CCK-8 assay; ( C ) the MAP-2 positive MNs, counting manually in different IL-17A treatments; ( D ) the neurite length of MAP-2 positive MNs was calculated using Neuron J in the FIJI software; ( E ) the immunostaining of MAP-2 with the same IL-17A treatment at different times (three days and six days); ( F ) the MAP-2 positive MNs’ counting in IL-17A treatment at different times; ( G ) the length of the neurite was measured under the same treatment. * p < 0.05, ** p < 0.01, *** p < 0.001 versus controls, ns: no significance.
Human Il 5 R Alpha/Cd125 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+il+33+neutralizing+antibody/Human+IL-5+R+alpha%2FCD125+Antibody/bio-techne+corporation___af-253-na
Average 90 stars, based on 1 article reviews
human il-5 r alpha/cd125 antibody - by Bioz Stars, 2026-10
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rabbit anti-rat il-2 neutralizing polyclonal antibody  (Becton Dickinson)
90
Becton Dickinson rabbit anti-rat il-2 neutralizing polyclonal antibody
Impact of <t>IL-17A</t> on MNs: ( A ) the MAP-2 immunostaining after different concentrations (5 ng/mL, 50 ng/mL, and 500 ng/mL) of IL-17A-stimulated MNs for three days; ( B ) the viability of MNs under the same IL-17A treatment for three days was evaluated using CCK-8 assay; ( C ) the MAP-2 positive MNs, counting manually in different IL-17A treatments; ( D ) the neurite length of MAP-2 positive MNs was calculated using Neuron J in the FIJI software; ( E ) the immunostaining of MAP-2 with the same IL-17A treatment at different times (three days and six days); ( F ) the MAP-2 positive MNs’ counting in IL-17A treatment at different times; ( G ) the length of the neurite was measured under the same treatment. * p < 0.05, ** p < 0.01, *** p < 0.001 versus controls, ns: no significance.
Rabbit Anti Rat Il 2 Neutralizing Polyclonal Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+il+33+neutralizing+antibody/rabbit+anti+rat+il+2+neutralizing+polyclonal+antibody/pm16371933-75-9-11
Average 90 stars, based on 1 article reviews
rabbit anti-rat il-2 neutralizing polyclonal antibody - by Bioz Stars, 2026-10
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anti-human il-1α neutralizing antibody  (Cosmo Bio USA)
90
Cosmo Bio USA anti-human il-1α neutralizing antibody
Impact of <t>IL-17A</t> on MNs: ( A ) the MAP-2 immunostaining after different concentrations (5 ng/mL, 50 ng/mL, and 500 ng/mL) of IL-17A-stimulated MNs for three days; ( B ) the viability of MNs under the same IL-17A treatment for three days was evaluated using CCK-8 assay; ( C ) the MAP-2 positive MNs, counting manually in different IL-17A treatments; ( D ) the neurite length of MAP-2 positive MNs was calculated using Neuron J in the FIJI software; ( E ) the immunostaining of MAP-2 with the same IL-17A treatment at different times (three days and six days); ( F ) the MAP-2 positive MNs’ counting in IL-17A treatment at different times; ( G ) the length of the neurite was measured under the same treatment. * p < 0.05, ** p < 0.01, *** p < 0.001 versus controls, ns: no significance.
Anti Human Il 1α Neutralizing Antibody, supplied by Cosmo Bio USA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+il+33+neutralizing+antibody/anti+human+il+1%CE%B1+neutralizing+antibody/pm12237882-31-0-12
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Image Search Results


Impact of IL-17A on MNs: ( A ) the MAP-2 immunostaining after different concentrations (5 ng/mL, 50 ng/mL, and 500 ng/mL) of IL-17A-stimulated MNs for three days; ( B ) the viability of MNs under the same IL-17A treatment for three days was evaluated using CCK-8 assay; ( C ) the MAP-2 positive MNs, counting manually in different IL-17A treatments; ( D ) the neurite length of MAP-2 positive MNs was calculated using Neuron J in the FIJI software; ( E ) the immunostaining of MAP-2 with the same IL-17A treatment at different times (three days and six days); ( F ) the MAP-2 positive MNs’ counting in IL-17A treatment at different times; ( G ) the length of the neurite was measured under the same treatment. * p < 0.05, ** p < 0.01, *** p < 0.001 versus controls, ns: no significance.

Journal: International Journal of Molecular Sciences

Article Title: Interleukin-17 and Th17 Lymphocytes Directly Impair Motoneuron Survival of Wildtype and FUS-ALS Mutant Human iPSCs

doi: 10.3390/ijms22158042

Figure Lengend Snippet: Impact of IL-17A on MNs: ( A ) the MAP-2 immunostaining after different concentrations (5 ng/mL, 50 ng/mL, and 500 ng/mL) of IL-17A-stimulated MNs for three days; ( B ) the viability of MNs under the same IL-17A treatment for three days was evaluated using CCK-8 assay; ( C ) the MAP-2 positive MNs, counting manually in different IL-17A treatments; ( D ) the neurite length of MAP-2 positive MNs was calculated using Neuron J in the FIJI software; ( E ) the immunostaining of MAP-2 with the same IL-17A treatment at different times (three days and six days); ( F ) the MAP-2 positive MNs’ counting in IL-17A treatment at different times; ( G ) the length of the neurite was measured under the same treatment. * p < 0.05, ** p < 0.01, *** p < 0.001 versus controls, ns: no significance.

Article Snippet: Further, 0.5 µg/mL of IL-17A neutralizing antibody (1:1,000, BPS Bioscience, San Diego, CA, USA) and 1µg/mL of human IL17RA/IL-17R antibody pretreatment (1:500, R&D Systems, Minneapolis, MN, USA) for 0.5 h prior to adding IL-17A was performed for three days.

Techniques: Immunostaining, CCK-8 Assay, Software

Effect of IL-17F on MNs: ( A ) immunostaining of MAP-2 in IL-17F (5 ng/mL, 50 ng/mL, and 500 ng/mL) stimulated MNs; ( B ) the viability of MNs under different IL-17F treatments was measured using CCK-8 assay; ( C ) MAP-2 positive MNs counting under different IL-17F treatments; (D) the neurite length was calculated, using Neuron J in the FIJI software, under different treatment conditions in both cells, ns: no significance.

Journal: International Journal of Molecular Sciences

Article Title: Interleukin-17 and Th17 Lymphocytes Directly Impair Motoneuron Survival of Wildtype and FUS-ALS Mutant Human iPSCs

doi: 10.3390/ijms22158042

Figure Lengend Snippet: Effect of IL-17F on MNs: ( A ) immunostaining of MAP-2 in IL-17F (5 ng/mL, 50 ng/mL, and 500 ng/mL) stimulated MNs; ( B ) the viability of MNs under different IL-17F treatments was measured using CCK-8 assay; ( C ) MAP-2 positive MNs counting under different IL-17F treatments; (D) the neurite length was calculated, using Neuron J in the FIJI software, under different treatment conditions in both cells, ns: no significance.

Article Snippet: Further, 0.5 µg/mL of IL-17A neutralizing antibody (1:1,000, BPS Bioscience, San Diego, CA, USA) and 1µg/mL of human IL17RA/IL-17R antibody pretreatment (1:500, R&D Systems, Minneapolis, MN, USA) for 0.5 h prior to adding IL-17A was performed for three days.

Techniques: Immunostaining, CCK-8 Assay, Software

Immunostaining of the IL-17 receptor (IL-17RA and IL-17RC) and MHCI in MNs: ( A ) immunostaining of IL-17RA, IL-17RC, and MHCI in FUS-WT-EGFP, FUS-P525L-EGFP, PBMCs, and a fibroblast: ( B ) co-staining of MAP-2 with IL-17RA, IL-17RC, and MHCI in FUS-WT-EGFP and FUS-P525L-EGFP cells; ( C ) the counting of IL-17RA positive cells in MNs; ( D ) IL-17RA positive staining in MNs; ( E ) MHCI positive staining in MNs. * p < 0.05 versus controls, ns: no significance.

Journal: International Journal of Molecular Sciences

Article Title: Interleukin-17 and Th17 Lymphocytes Directly Impair Motoneuron Survival of Wildtype and FUS-ALS Mutant Human iPSCs

doi: 10.3390/ijms22158042

Figure Lengend Snippet: Immunostaining of the IL-17 receptor (IL-17RA and IL-17RC) and MHCI in MNs: ( A ) immunostaining of IL-17RA, IL-17RC, and MHCI in FUS-WT-EGFP, FUS-P525L-EGFP, PBMCs, and a fibroblast: ( B ) co-staining of MAP-2 with IL-17RA, IL-17RC, and MHCI in FUS-WT-EGFP and FUS-P525L-EGFP cells; ( C ) the counting of IL-17RA positive cells in MNs; ( D ) IL-17RA positive staining in MNs; ( E ) MHCI positive staining in MNs. * p < 0.05 versus controls, ns: no significance.

Article Snippet: Further, 0.5 µg/mL of IL-17A neutralizing antibody (1:1,000, BPS Bioscience, San Diego, CA, USA) and 1µg/mL of human IL17RA/IL-17R antibody pretreatment (1:500, R&D Systems, Minneapolis, MN, USA) for 0.5 h prior to adding IL-17A was performed for three days.

Techniques: Immunostaining, Staining

Antagonizing IL17 protects from IL17-induced neurodegeneration: ( A ) IL-17A-neutralizing pretreatment for 0.5 h, 50 ng/mL IL-17A stimulated the MNs for three days, MAP-2 immunostaining was performed after the treatment; ( B ) the viability of MNs under the same treatment mentioned before was calculated using CCK-8 assay; ( C ) MAP-2 positive MNs were counted and (D) the neurite length of MNs were measuredunder different treatments; ( E ) immunostaining of MAP-2 under anti-IL-17RA/IL-17R receptor pretreatment for 0.5 h, then 50 ng/mL IL-17A-stimulated for three days; ( F ) viability of MNs; ( G ) MAP-2 positive MNs counting; (H) neurite length of MAP-2 positive MNs using the aforementioned treatment.* p < 0.05, ** p < 0.01 versus controls, ns: no significance.

Journal: International Journal of Molecular Sciences

Article Title: Interleukin-17 and Th17 Lymphocytes Directly Impair Motoneuron Survival of Wildtype and FUS-ALS Mutant Human iPSCs

doi: 10.3390/ijms22158042

Figure Lengend Snippet: Antagonizing IL17 protects from IL17-induced neurodegeneration: ( A ) IL-17A-neutralizing pretreatment for 0.5 h, 50 ng/mL IL-17A stimulated the MNs for three days, MAP-2 immunostaining was performed after the treatment; ( B ) the viability of MNs under the same treatment mentioned before was calculated using CCK-8 assay; ( C ) MAP-2 positive MNs were counted and (D) the neurite length of MNs were measuredunder different treatments; ( E ) immunostaining of MAP-2 under anti-IL-17RA/IL-17R receptor pretreatment for 0.5 h, then 50 ng/mL IL-17A-stimulated for three days; ( F ) viability of MNs; ( G ) MAP-2 positive MNs counting; (H) neurite length of MAP-2 positive MNs using the aforementioned treatment.* p < 0.05, ** p < 0.01 versus controls, ns: no significance.

Article Snippet: Further, 0.5 µg/mL of IL-17A neutralizing antibody (1:1,000, BPS Bioscience, San Diego, CA, USA) and 1µg/mL of human IL17RA/IL-17R antibody pretreatment (1:500, R&D Systems, Minneapolis, MN, USA) for 0.5 h prior to adding IL-17A was performed for three days.

Techniques: Immunostaining, CCK-8 Assay